M.-T. Riou & E. Jaspard

• Derivatization of individual amino acids with phenylisothicyanate (PITC)
• Extraction of amino acids from cotyledons and axis and derivatization with PITC
• HPLC system

II. Separation of the phenylthiocarbamyl (PTC) amino acids
 

Chromatogram of the separation of 21 PTC amino acids Summary of parameters of standard curves for 21 PTC amino acids Example of standard curve : PTC - Asp & PTC - Glu Example of standard curve : PTC - Arg & PTC - Lys Example of standard curve : PTC - Asn & PTC - Gln Comparison of separation of PTC - amino acids

III. Results (standard error bars in the figures correspond to n = 5 to 11 different injections)
 

Imbibition & germination curves of Pisum sativum seeds Separation of amino acids from a biological sample Asp / Glu / Arg / Lys Phe / Tyr / Trp / His Gln / Asn / Gly / Pro Thr / Ser / Met Ala / Val / Ile / Leu

IV. Global analysis of all individual amino acids

 

Analysis Interpolation Decomposition

IV. Conclusion

V. References